Intermediate

Part:BBa_K539627:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0032)+Alss


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 327
    Illegal BsaI.rc site found at 921


Design Notes

Built in weak expressing RBS (Part:BBa_B0032), and Alss (Part:BBa_K539713). The sequence of Alss had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

The Alss sequence comes from Bacillus subtilis.

References